Proliferating cell nuclear antigen (P04961)

Uniprot ID P04961
Protein Name Proliferating cell nuclear antigen
Gene Name Pcna
Species Rattus norvegicus (Rat)
Signal peptide(a) N Secretome P(b)
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). .
GO - Molecular function
  • chromatin binding : ISS:UniProtKB
  • damaged DNA binding : ISS:UniProtKB
  • dinucleotide insertion or deletion binding : ISO:RGD
  • DNA polymerase binding : ISO:RGD
  • DNA polymerase processivity factor activity : IBA:GO_Central
  • enzyme binding : ISO:RGD
  • estrogen receptor binding : IPI:RGD
  • histone acetyltransferase binding : ISO:RGD
  • identical protein binding : ISO:RGD
  • MutLalpha complex binding : ISO:RGD
  • protein C-terminus binding : IEA:Ensembl
  • purine-specific mismatch base pair DNA N-glycosylase activity : ISO:RGD
  • receptor tyrosine kinase binding : ISO:RGD
  • transcription factor binding : ISO:RGD
GO - Biological process
  • base-excision repair, gap-filling : ISO:RGD
  • cellular response to hydrogen peroxide : IEP:RGD
  • cellular response to UV : IEP:RGD
  • cellular response to xenobiotic stimulus : ISO:RGD
  • epithelial cell differentiation : ISO:RGD
  • estrous cycle : IEP:RGD
  • heart development : IEP:RGD
  • leading strand elongation : IBA:GO_Central
  • liver regeneration : IEP:RGD
  • mismatch repair : ISO:RGD
  • mitotic telomere maintenance via semi-conservative replication : ISO:RGD
  • negative regulation of transcription by RNA polymerase II : ISO:RGD
  • positive regulation of deoxyribonuclease activity : ISS:UniProtKB
  • positive regulation of DNA repair : ISS:UniProtKB
  • positive regulation of DNA replication : ISS:UniProtKB
  • positive regulation of DNA-directed DNA polymerase activity : IEA:Ensembl
  • replication fork processing : ISO:RGD
  • response to cadmium ion : IEP:RGD
  • response to dexamethasone : IEP:RGD
  • response to estradiol : IEP:RGD
  • response to L-glutamate : IEP:RGD
  • response to lipid : IDA:RGD
  • response to oxidative stress : IEP:RGD
  • translesion synthesis : ISS:UniProtKB
(a) The Signal peptide D-score cutoff for "YES"(having signal peptide) is 0.45.
(b) Non-classically secreted proteins should obtain an NN-score(Neural Networks score) exceeding the normal threshold of 0.5, but not at the same time be predicted to contain a signal peptide.