Glyceraldehyde-3-phosphate dehydrogenase (P04797)

Uniprot ID P04797
Protein Name Glyceraldehyde-3-phosphate dehydrogenase
Gene Name Gapdh
Species Rattus norvegicus (Rat)
Signal peptide(a) N Secretome P(b)
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). .
GO - Molecular function
  • aspartic-type endopeptidase inhibitor activity : ISO:RGD
  • disordered domain specific binding : ISO:RGD
  • enzyme binding : ISO:RGD
  • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity : IDA:UniProtKB
  • identical protein binding : IPI:RGD
  • microtubule binding : IDA:UniProtKB
  • NAD binding : IEA:InterPro
  • NADP binding : IEA:InterPro
  • peptidyl-cysteine S-nitrosylase activity : IDA:UniProtKB
GO - Biological process
  • antimicrobial humoral immune response mediated by antimicrobial peptide : ISO:RGD
  • apoptotic process : IMP:RGD
  • cAMP-mediated signaling : IMP:RGD
  • carbohydrate metabolic process : IDA:RGD
  • cellular response to interferon-gamma : ISO:RGD
  • defense response to fungus : ISO:RGD
  • female pregnancy : IEP:RGD
  • gluconeogenesis : IDA:RGD
  • glycolytic process : IDA:RGD
  • killing by host of symbiont cells : ISO:RGD
  • killing of cells of other organism : ISO:RGD
  • microtubule cytoskeleton organization : IDA:UniProtKB
  • multicellular organism development : ISO:RGD
  • negative regulation of endopeptidase activity : ISO:RGD
  • negative regulation of translation : ISO:RGD
  • negative regulation of vascular associated smooth muscle cell apoptotic process : IMP:RGD
  • neuron apoptotic process : IDA:UniProtKB
  • peptidyl-cysteine S-trans-nitrosylation : IDA:UniProtKB
  • positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction : ISO:RGD
  • positive regulation of cytokine secretion : ISO:RGD
  • protein stabilization : IDA:UniProtKB
  • response to ammonium ion : IDA:RGD
(a) The Signal peptide D-score cutoff for "YES"(having signal peptide) is 0.45.
(b) Non-classically secreted proteins should obtain an NN-score(Neural Networks score) exceeding the normal threshold of 0.5, but not at the same time be predicted to contain a signal peptide.